![]() Andralojc KM, Campbell AC, Kelly AL, Terrey M, Tanner PC, Gans IM, Senter-Zapata MJ, Khokhar ES, Updike DL.PQN-75 is expressed in the pharyngeal gland cells of Caenorhabditiselegans and is dispensable for germline development. Rochester JD, Tanner PC, Sharp CS, Andralojc KM, Updike DL. ![]() Membraneless organelles: P granules in Caenorhabditis elegans. Germline Maintenance Through the Multifaceted Activities of GLH/Vasa in Caenorhabditis elegans P Granules. Marnik EA, Fuqua JH, Sharp CS, Rochester JD, Xu EL, Holbrook SE, Updike DL.Quelling germ cell pluripotency on the genital ridge.Reed, K.J., Svendsen, J.M., Brown, K.C., Montgomery, B.E., Marks, T.N., Vijayasarathy, T., Parker, D.M., Osborne-Nishimura, E., Updike, D.L., Montgomery, T.A., Nucleic Acids Research. Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans.We are continuing to investigate the specifics of those processing events to understand how they are used to maintain the pluripotent and immortal properties of germ cells, and how these mechanisms can be applied to unlocking stem cell-like properties in other cell types. Here germ granules create a specialized microenvironment that facilitates post-transcriptional processing events that are exclusive to the germline. We have shown that germ granules are held together by weak hydrophobic interactions, and that they extend the size exclusion barrier of the nuclear pore complex out into the cytoplasm of germ cells. elegans combined with its short 3-day generation time and transparency offers an opportunity to study the function of germ granules that isn’t feasible in more complex model organisms.Ī focus of our research is to understand the biophysical properties of germ granules, how they are assembled, and the components that affect their subcellular distribution. ![]() elegans) can be observed in the germ line of transparent worms at all stages of development. Using fluorescence microscopy, germ granules (called P granules in C. This well-established model organism is responsible for much of what we know about developmental genetics in humans. Because of this, we are able to quickly ascertain the mechanisms by which germ granules function by studying them in a small roundworm called C. Germ granules are conserved in their appearance, subcellular localization, and composition from worms to humans. In some organisms, introducing germ granules in cells outside of the germline will restore cellular immortality and totipotency, and will reprogram them into germline stem cells. Take away an organism’s germ granules and it will become sterile. Recent research has shown that germ granules play a critical role in maintaining the totipotent and immortal properties of the germline. Part of the answer comes from special aggregates called “germ granules” that are found just outside the nucleus of germ cells. In our lab, we want to understand how these totipotent and immortal properties are conferred. Germ cells are the progenitors of reproductive cells, and the only cells in sexually reproducing animals that have the potential to give rise to all of the cell types of each subsequent generation.
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